Center for Analytical Instrumentation Development
CAID Second Annual Meeting
June 16 and 17, 2009
Meeting Schedule

CAID Annual Meeting
June 16 and June 17, 2009
Purdue University


Tentative Schedule

Tuesday, June 16, 2009
10am - 3pm
Wetherill Hall,
First Floor Lobby
Lab Tours and Demos (optional)
3:00 pm
Wetherill Hall,
Room 104
Plenary Session I
Introduction
- Prof. Graham Cooks, CAID, Purdue University

Issues and Direction is Biological and Pharmaceutical Analysis
- Prof. Fred Regnier, CAID, Purdue University

From Ambient Tissue and Single Cell Analysis by LAESI to Nano-photonic Ion Sources
- Prof. Akos Vertes, George Washington University

Exploring the use of Desorption Electrospray Ionization (DESI) for Direct Measurements of Xenobiotics on
Paper Surfaces and in Tissue Sections

- Dr. Justin Wiseman, Prosolia, Inc.

The following Tuesday evening events will be held at Thomas Duncan Hall, 619 Ferry Street, Lafayette, Indiana:

5:30 pmReception
6:30 pmDinner
7:30 pmKeynote Address
The Use of Dried Blood Spots Samples for the Quantitative Bio-analysis of Drugs - Opportunities for
Direct Analysis

- Dr. Neil Spooner, GlaxoSmithKline
8:30 pmPanel Discussion

Wednesday, June 17, 2009
9:00 am
Wetherill Hall,
Room 104
Plenary Session II
Ambient Ionization Methods Coupled to FT-MS for Direct Analysis and Imaging Applications
- Prof. David Muddiman, North Carolina State University

Ambient Imaging Using Desorption Electrospray Ionization-Mass Spectrometry (DESI-MS)
- Dr. Demian Ifa, Purdue University

Unraveling the Secrets of the Brain with New Analytical Techniques
- Prof. Jonathan Sweedler, University of Illinois Urbana-Champaign
11:00 am
Wetherill Hall,
Room 104
Panelon Instrumentation
12:00 pmAdjourn
1pm - 5pmLab Tours and Demos (optional)

Speaker Biographies

The general theme will be the analysis of pharmaceuticals in dried blood spots. Our speakers are renowned experts in mass spectrometry and analytical chemistry. Talks will be on new analytical methods for interrogating biological samples and tissue imaging.


Keynote Address: The Use of Dried Blood Spots Samples For The Quantitative Bioanalysis of Drugs - Opportunities For Direct Analysis: Dr. Neil Spooner, GlaxoSmithKline • Dr. Neil Spooner is Director of the Bioanalytical Sciences and Development group of the Worldwide Bioanalysis and Systems Management Department of DMPK at GlaxoSmithKline Pharmaceuticals R & D, based in Ware, UK. He is responsible for the investigation, evaluation and implementation of technologies and processes to enhance and revolutionize the way that the quantitative bioanalysis of drugs is performed. He obtained his B.Sc. (Hons) and Ph.D. qualifications in Biochemistry from the University of Liverpool and performed post doctoral studies on LC-MS/MS at the University of Bristol. Dr. Spooner has over 15 years of experience in the quantitative bioanalysis of drugs in biological fluids and has worked in a variety of managerial and lab based roles in the UK and USA. He has taken a leadership role in the evaluation and global implementation of dried blood spot technology as the first choice technique for the collection of samples for quantitative drug bioanalysis in pre-clinical and clinical studies at GlaxoSmithKline.

Ambient Ionization Methods Coupled to FT-MS for Direct Analysis and Imaging Applications: Prof. David Muddiman, North Carolina State University • Dr. David C. Muddiman is Professor of Chemistry and Director of both the W.M. Keck FT-ICR Mass Spectrometry Laboratory and the Mass Spectro-metry Facility at NCSU in Raleigh, NC. He received his B.S. from Gannon University (Erie, PA) in 1990 and his Ph.D. in Analytical Chemistry from the Univer-sity of Pittsburgh with post-doctoral work at Pacific Northwest National Lab. His research focuses on new ionization methods, FT-ICR fundamentals, and alternative splicing databases. These developments are directed at solving problems including ovarian cancer, congestive heart failure and biofuels. Dr. Muddiman serves on the advisory board of the National Science Foundation FT-ICR Mass Spectrometry Laboratory of the National High Magnetic Field Laboratory, as well as several editorial boards for journals in analytical chemistry. Among his awards are the Arthur F. Findeis Award from the American Chemical Society and American Society for Mass Spectrometry Research Award.

From Ambient Tissue and Single Cell Analysis by LAESI to Nanophotonic Ion Sources: Prof. Akos Vertes, George Washington University • Dr. Akos Vertes is Professor of Chemistry, Biochemistry and Molecular Biology at the George Washington University in Washington, DC. He is co-director of the W. M. Keck Institute for Proteomics Technology and Applications at the university. Dr. Vertes received B.S. and Ph.D. degrees from Eotvos Lorand University in Budapest, Hungary, in 1974 and 1979 respectively. Dr. Vertes’ research interests span from fundamental studies in analytical and physical chemistry to the development of new technologies for biomedical analysis. Recent accomplish-ments include the introduction of photonic ion sources based on nanostructures (e.g., laser-induced silicon microcolumn arrays or LISMA), a new ambient ion source, laser ablation electrospray ionization (LAESI), metabolic imaging of live plant organs and in situ mass spectrometric analysis of single cells. Honors and awards include Doctor of the Hungarian Academy of Sciences and the Elsevier Spectrochimica Acta Award.

Unraveling the Secrets of the Brain with New Analytical Techniques: Prof. Jonathan Sweedler, University of Illinois at Urbana-Champaign • Dr. Jonathan V. Sweedler holds the James R. Eiszner Family Chair in Chemistry at the University of Illinois and is Director of the UIUC Biotechnology Center. He is associated with the Beckman Institute and also has appointments in Neuroscience, Physiology and Bioengineering. Professor Sweedler received his B.S. degree from the University of California-Davis in 1983 and his Ph.D. from the University of Arizona in 1989, with post-doctoral work at Stanford University. His research interests are in bioanalytical chemistry and focus on new metabolomic and peptidomic technologies for assaying small volume samples and their application to neurochemistry. Technologies include nanoliter volume NMR, single-cell mass spectrometry, information rich spectroscopic detectors for capillary-scale separations, and hybrid nanofluidic/microfluidic devices for neuronal sampling. He is investigating novel neurochemical path-ways and the roles that peptide hormones, neurotransmitters and neuromodulators play in behavior, learning and memory. Among his awards is the American Chemical Society’s Instrumentation Award.

Exploring the use of Desorption Electrospray Ionization (DESI) for Direct Measurements of Xenobiotics on Paper Surfaces and in Tissue Sections: Dr. Justin Wiseman, Prosolia, Inc. • Dr. Justin M. Wiseman is currently Director of Research and Development at Prosolia, Inc., an Indianapolis, Indiana based start-up company specializing in the commercialization of desorption electrospray ionization (DESI) and related mass spectrometry techniques. He is a co-inventor of DESI. Dr. Wiseman has a B.S. from the University of Indianapolis and obtained a Ph.D. in Analytical Chemistry from Purdue University. Prior to obtaining his Ph.D., he worked at Eli Lilly & Co.in Indianapolis, IN as an analytical chemist in support of parenteral manufacturing. While at Purdue, he completed a two year NSF fellowship, received the 2006 Schering-Plough Science and Innovation Award, and obtained a certificate in business management from the Krannert School of Management under the Applied Management Principles program. Dr. Wiseman’s research interests include atmospheric desorption/ ionization techniques and its applications in bioanalytical chemistry and forensics, imaging mass spectrometry, and microfluidics. Special interests include pharmacokinetics/toxicokinetics of xenobiotics.

Ambient Imaging Using Desorption Electrospray Ionization—Mass Spectrometry (DESI-MS): Dr. Demian Ifa, Purdue University • Dr. Demian Ifa is a research scientist at Purdue University in Prof. Graham Cooks’ laboratory. There, Dr. Ifa is focused on developing new ambient ionization techniques to image the distribution of compounds in biological tissues and on surfaces. Such technologies will have important applications in biology such as tumor tissue discrimination as well as in forensic science, e.g. the determination of illicit material in fingerprints. Dr. Ifa graduated from the State University of Sao Paulo, Brazil in 1995 with a B.S. in pharmaceutical sciences. He obtained an M.S. in Organic Chemistry from Federal University in Rio de Janeiro in 1998; and a Ph.D. in Pharmacology from Sao Paulo University in 2002. He has extensive experience in the development of novel mass spectrometric techniques to interrogate analytical and biological problems. His experience includes drug/metabolite quantification in complex biological matrices, elucidation of protein structure and imaging of biological tissues using mass spectrometry.

Issues and Directions in Biological and Pharmaceutical Analysis: Prof. Fred Regnier, Purdue University • Dr. Fred Regnier is J.H. Law Distinguished Professor of Chemistry and co-director of CAID at Purdue University. Dr. Regnier received his Bachelor’s degree in 1960 from Nebraska State College and the Ph.D. from Oklahoma State University in 1965, conducting post-doctoral work at the University of Chicago and Harvard. His research interests include developing integrated analytical systems for the analysis and characterization of complex protein mixtures using multidimensional separation systems and mass spectrometry. Current work is centered on identifying proteins from cellular extracts that are in regulatory flux. A wide variety of stimuli are being examined ranging from cancer to specific diseases. Dr. Regnier is also an accomplished entrepreneur, co-founding such companies as PerSeptive Biosystems now part of Applied Biosystems, Beyond Genomics, which provides advanced drug discovery tools to the pharmaceutical and biotechnology industries, and Quadraspec. He has received numerous awards and distinctions for his research in chemistry and biochemistry, including the Purdue University Outstanding Commercialization Award and the Chromatography Award from the American Chemical Society.


About the Center for Analytical Instrumentation Development:  Analytical Chemistry and its instruments provide the measurement and quantitative information underlying much of the research and commercial activity in chemistry, biology, and medicine. The Center for Analytical Instrumentation Development (CAID) has been created in Discovery Park to create the next generation of “machine tools for science” and to enhance the role of analytical chemistry in building the infrastructure of the nation, by developing new instrumentation with broad applicability. Areas of societal importance are being addressed, including clinical diagnostics and medical testing, neurobiology, climate change, food safety, and national security. Participating institutions are Purdue, Indiana University, the University of Illinois-Urbana-Champaign, and the University of Notre Dame, building on the tremendous regional strength in analytical chemistry.