Center for Analytical Instrument Development

2013 Annual Meeting

Intensive Short Course on Mass Spectrometry
for Undergraduates

Undergraduates

Devin Bready, Dalton Snyder, Christina Schilling, Chase Freeman, Angela Silski, Jessica Bosse

Synthesis by Mass Spectrometry

September 22 & 23, 2013

During the two day short course, undergraduates from different universities performed a traditional bulk phase reaction and preparative mass spectrometry via paperspray in a laboratory setting.

 

Experiments

Three organic reactions, Claisen-Schmidt base-catalyzed condensation, Hantsch synthesis of symmetric dihydropyridines, and Girard's reagent T condensation with hydrocortisone, were performed in both a bulk reaction and via the accelerated mass spectrometry method of paperspray. The reaction in bulk took approximately 30 minutes for completion in comparison to a few seconds via paperspray. Micro-droplets from the paperspray produce product due to le Chatlier's Principle, while in bulk reaction there are no micro-droplets formed. Students also adapted fruit spray from a previous experiment performed in Aston Labs, where they sprayed directly from the fruit into the instrument.

Paperspray

Paperspray relation to droplet size (in microns), "Spray 
mechanism in paper spray ionization" by Ryan Espy, Aston 
Labs Graduate Student

LTP

Fruit spray analyzed, detecting m/z of 297, "Screening of agrochemicals in foodstuffs 
using low-temperature plasma (LTP) ambient ionization mass spectrometry" by Josh 
Wiley, Aston Labs Graduate Student

Sunday, September 22

A short introduction to the intensive short course was given. Students performed the three bulk phase reactions and preparative mass spectrometry via paperspray. Discussion of the history of mass spectrometry and where we are today was given by Professor R. Graham Cooks. Fruit spray was also analyzed to detect different types of produce. Students were given an unknown mixture of two compounds, where they analyzed the solution via paperspray and used the spectra to determine the two chemical compounds in the mixture.

 

Monday, September 23

A tour of Aston Labs was given after a breakfast with the graduate students. After touring the labs, the students observed a demo, Desorption Electrospray Ionization (DESI) Mass Spectrometry Imaging: A Practicum in Molecular Discrimination of Diseasegiven by fellow graduate student Kevin Kerian. After this demonstration, the students registered for the Center of Analytical Instrument Development (CAID), where a brief introduction was given by Professor R. Graham Cooks. Students participated in a variety of demonstrations, where faculty and employers from different universities and companies introduced new and innovative analytical techniques, focusing on personalized medicine. 

KAREN!!!!!!!!!!!!!!!!!!!!!

Students from the undergraduate program listen to a lecture of a demo during CAID

Future Objectives

The short course is intended to grow as more students from different universities attend. The intention is to have students bring their own interesting experiments and reactions involving analytical instrumentation to share in a similar manner to CAID. Anyone interested in learning more about the undergraduate seminar can contact Brandy McMasters at mcmasters@purdue.edu

Contact

Co-Directors:
Professor Graham Cooks
560 Oval Drive
Department of Chemistry, Purdue University,
West Lafayette, IN 47907-1393
Phone: 765-494- 5263

Professor Fred Regnier
560 Oval Drive
Department of Chemistry, Purdue University,
West Lafayette, IN 47907-1393
Phone:765-494-3878

Bindley Bioscience Building
1203 W State Street
West Lafayette, IN 47907-2057
765-494-5262