Bindley Bioscience Center

Biomolecular Technologies

Overview :: Proteomics :: Metabolomics :: Genomics :: Ionomics
Resources/Infrastructure

Overview

OverviewAccess to the Biomolecular Technologies core of Bindley Bioscience Center is available to researchers whose projects require biomolecular analyses. These technologies are integrated and accessible at any level of involvement that is appropriate, from research collaborations to fee-for-service analyses. State-of-the-art instrumentation is available for genomic, metabolomic, and proteomic analyses. Led by expert Bindley Research Scientists, the Biomolecular Technologies core provides advice and direction both to investigators who have not previously employed these approaches in their research and to students and staff of Purdue research labs in biomolecular technical operations, data analysis and strategies to enhance the success of their projects. The four sub-cores of technologies include proteomics, metabolomics, genomics, and ionomics.

Proteomics: The Proteomics Core contains the high-throughput protein production equipment required for selecting soluble protein species for functional or structural studies including the equipment needed for scale-up to produce the selected proteins. Proteomics Facilities at the Bindley Bioscience Center provide the research community with state-of-the-art technologies to develop new methods and enable resources for providing proteomics analysis using both two-dimensional gel and multi-dimensional liquid chromatography separation coupled with mass spectrometry. The Proteomics facilities are composed of several major operations that include a variety of capabilities for protein analysis and expertise complimentary to existing Purdue University capabilities to enhance life sciences research on campus and in the region.

Metabolomics: The Metabolomics Core is composed of several major operations that involve a variety of expertise for metabolomic analysis. Metabolomics facilities at Bindley Bioscience Center provide the research community with state-of-the-art technologies to develop new methods, enable resources, and provide service for both "Qualitative" and "Quantitative Metabolomics" analysis using mass spectrometry coupled with gas and liquid chromatography (GC-MS and LC-MS). Each laboratory provides complementary expertise that globally serves to enhance the research capabilities of the Life Science research community on campus and in the region. The Metabolomics Core has the chromatographic and mass spectrometric equipment that is needed for analysis of small molecule metabolites from biological sources.

Genomics: The Genomics Core contains equipment for high-throughput DNA sequencing, high-accuracy DNA sequencing, and the appropriate computational software for analysis of the data. The Custom Genomic Facility (CGF) supports genomic research through the development and utilization of specific DNA microarray tools. Activities include the production of custom oligo-probe and cDNA-probe microarrays in support of gene expression profiling and SNP assays, as well as specific probe types such as proteins, antibodies and FRET-hairpin probes for use in mass spectrometry applications, capturing known proteins, and the detection of unlabeled genetic material, respectively. The CGF contains equipment for high-throughput DNA sequencing, high-accuracy DNA sequencing, and the appropriate computational software for analysis of the data. "Gene chip" technology for transcriptional analyses is also available in the Genomics Core. Goals include integration of high-throughput microarray-based transcript profiling and proteomic approaches to characterize molecular responses on a genomic scale. Data generated from experimental methodologies can be supported by the Computational Life Sciences & Informatics capabilities at the Bindley Bioscience Center to construct and develop predictive models of gene regulatory networks.

Ionomics: The advent of high-throughput phenotyping technologies has created a deluge of information that is difficult to deal with without the appropriate data management tools. These data management tools should integrate defined workflow controls for genomic-scale data acquisition and validation, data storage and retrieval, and data analysis indexed around the genomic information of the organism of interest. To maximize the impact of these large data sets, it is critical that they are rapidly disseminated to the broader research community, allowing the open access for data mining and discovery. The Purdue Ionomics Information Management System (PiiMS) is a system that incorporates such functionalities developed around the Purdue University high-throughput ionomics phenotyping platform. The PiiMS provides integrated workflow control and data storage/ analysis to facilitate high-throughput data acquisition, along with integrated tools for data search, retrieval and visualization for hypothesis development. PiiMS is deployed as a world-wide-web enabled system, allowing for the integration of distributed workflow processes. The open access to the raw data for analysis by numerous laboratories. PiiMS currently contains data on the shoot concentrations of P, Ca, K, Mg, Cu, Fe, Zn, Mn, Co, Ni, B, Se, Mo, Na, As, and Cd, in over 60,000 shoot tissue samples of Arabidopsis thaliana, including EMS, fast neutron and defined T-DNA mutants, natural accession and populations of recombinant inbred lines, from over 800 separate experiments, representing over 1,000,000 fully quantitative elemental concentrations. PiiMS is accessible at: http://www.purdue.edu/dp/ionomics/.

Center for Analytical Instrumentation Development (CAID) – Bindley Bioscience Center Room B019
Prof. Robert Cooks cooks@purdue.edu,  Prof. Fred Regnier fregnier@purdue.edu
CAID brings together chemists, physicist, engineers, biologist, and physicians from multiple Schools at Purdue University and other institutions in the region. The center develops innovative “machine-tools of science” that enable discoveries across a broad spectrum of life science. These activities will lead to routing, point-of-need devices for use in drug discovery, clinical diagnostics, environmental monitoring, and the fight against chemical and biological terrorism.
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Resources and Infrastructure

For detailed information about the Biomolecular Core facilities and laboratories, please click on the links below:

Purdue Proteomics Facility (PPF)

Metabolomics Profiling Facility (MPF)

Integrated Screening Technologies (IST)

Purdue Ionomics Information Management System (PiiMS)

Custom Genomics Facility (CGF)

Biopolymer Synthesis Laboratory

Biophysical Analysis Laboratory

Core Technologies

Biomolecular Biomolecular Technology Comp. Life Sciences & Informatics Cytomics & Imaging

Contact Information

Bindley Contact
Tommy Sors, Ph.D
tsors@purdue.edu
Office 49-41678
BIND 208

Faculty Contact
Joseph Irudayaraj, Ph.D.
josephi@purdue.edu
Office 49-40388
ABE 215

About Bindley

Interdisciplinary life sciences and engineering researchers collaborate to explore new technologies and scientific knowledge that impact the broad boundaries of plant, animal, and human diseases.


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Bindley Bioscience Center
1203 W. State Street
Purdue University
West Lafayette, IN 47907-2057