Bindley Bioscience Center

Instruments

Applied BioSystems 4800

The advantages of MALDI-TOF TOF include sensitivity into the picomole range and high mass accuracy. In addition, since there is usually only a singly charged ion formed, it is a good choice for the analysis of heterogeneous samples. Furthermore, the static nature of the sample allows for multiple evaluations on a single sample.

Nano LC-high resolution hybrid ion trap (Thermo Orbitrap)

The Orbitrap is a sub-dalton-resolution proprietary ion trap configuration instrument that enables very high resolution analysis of peptides, post-translationally modified peptides as well as small molecule detection and quantification. The Orbitrap rivals FT-ICR mass spectrometers with an easy to use and easy to maintain setup.

NanoLC-ion trap Analysis (Agilent XCT plus)

The advantage of the ESI-ion trap is that the ESI source can generate multiple charge states allowing for increase mass range and the ion trap can perform multiple MS/MS scan with rapid scanning speed and very high sensitivity.  The XCT plus is typically coupled to the Chip Cube system for low sample volume analyses.

NanoLC-Triple quadrupole mass spec (Agilent 6410)

The Agilent 6410 triple quad provides high level sensitivity for selected ion monitoring and multiple reaction monitoring for biomarker quantification studies. The 6410 includes both ESI and photoionization capability and has an on-board Chip Cube nanoLC system.

NanoLC-TOF (Agilent MSD TOF)

The advantage of the  ESI TOF is that it utilizes both the capacity of ESI to generate multiple charge states and extend the mass range of  the spectrometer by reducing the m/z ratio of large ions and the high resolution and mass acuracy afforded the TOF instruments. This is particluarly useful when preforming PMF studies with complexed mixtures.  Furthermore, the Agilent MSD TOF can be coupled to the Chip cube.

NanoLC-TOF and NanoLC-Ion trap combined platform (Agilent MSD TOF, XCT plus ion trap and 6410 triple quadrupole)

Combing these Agilent instruments into one platform results in high resolution peak picking followed by rapid, sensitive MS/MS identification and highest sensitivity peptide monitoring with MSn capabilities on the triple quad.  The modular Chip Cube can be coupled to all three machines for identical LC conditions that enable reproducible analysis. The chip cube also allows dramatic reduction of starting material and therefore the possibility to use difficult to obtain low volume samples for deep proteome interrogation.

 


PROTEOMICS SUBMISSION FORM

Contact

Address

Purdue University
Purdue Proteomics Facility (PPF)
Bindley Bioscience Center
1203 W. State St.
West Lafayette, IN 47907-2010

 

Lake Paul

Lake Paul, PhD

Facility Manager

Vicki Hedrick

Vicki Hedrick

Research Assitant