Highlighted below are the major research instruments incorporated by the Metabolite Profiling Facility for small molecule metabolite analysis.
Waters Micromass LCT Premier (TOF)
Waters Micromass LCT Premier (TOF), coupled to HPLC. The instrument is an easy to use benchtop mass spectrometer that utilizes a high-resolution time-of-flight (TOF) analyzer to enable automated exact mass measurements. The TOF analyzer utilizes W-Optics, a novel method for enhancing resolution that provides up to 10,000 FWMH resolution. The use of in-source collision induced dissociation (CID) can also yield structural information. This system is coupled with a Waters CapLS System cap/nano 1-D HPLC system capable of microliter and nanoliter flow rates.Similarly, the MS is equipped with regular and nano electrospray ionization sources.
Waters Micromass Q-Tof micro
Waters Micromass Q-Tof micro, coupled to HPLC. The instrument is a high resolution time-of-flight (TOF) mass spectrometer that enables automated exact mass measurements. The instrument features a quadrupole mass filter and collision cell prior to the TOF for MS/MS analyses. This powerful combination delivers exact mass measurement of fragment ions to yield increased confidence in structural elucidation and database searching. This instrument is coupled with a Waters 2795 Alliance HPLC system, with autosampler and photodiode array (PDA) detector. The MS is equipped with electrospray and APCI ionization sources.
Agilent MSD/TOF, coupled to HPLC. The instrument utilizes an innovative analog-to-digital (ADC) acquisition system that offers high resolution mass accuracy over a wide dynamic range. The automated calibrant delivery system and dual-nebulizer electrospray ion source of the LC/MSD TOF can continuously introduce reference mass compounds at very low levels. This results in outstanding mass accuracy, typically less than 3 ppm. This instrument is coupled with an Agilent 1100HPLC system, with autosampler and photodiode array (PDA) detector. Two such instruments are available to the MPF.
Agilent 1100 HPLC/UV-vis/Fluorescence
A stand-alone Agilent 1100 HPLC system supports both ultraviolet and fluorescence detection. The HPLC pump is a binary system capable of up to a 10 ml/min flow rate and 400 bar of pressure. The UV-vis detector is a diode array, with a wavelength range from 190 – 950 nm. The fluorescence detector provides simultaneous quantitative and qualitative information for up to four signals simultaneously. The system also incorporates a mobile phase degasser, column heater, and 100 vial autosampler. Data acquisition and analysis is performed using ChemStation software.
LECO Pegasus 4D GCxGC-TOF
LECO Pegasus 4D GCxGC-TOF. This instrument can be operated as either a GC/MS or a GCxGC/MS. When used in GCxGC mode, it is possible to separate and detection hundreds-to-thousands of compounds in a single sample. This would not be possible by conventional GC/MS. Separations, which previously resulted in dozens or hundreds of peaks, now yield thousands of individual components from complex mixtures. The powerful, easy-to-use ChromaTOF® software simplifies component identification by employing integrated NIST database searches. Other unique processing capabilities (such as sample comparisons) allow for automated mining of complex GCxGC data sets to extract previously unidentifiable similarities and differences. Ionization is performed using electron impact (EI). The upper mass limit for this instrument is 1,000 daltons. Different derivitization schemes are routinely employed to asssit in sample volatility. Two such instruments are available to the MPF.
Agilent 6460 Triple Quadrupole
The Agilent 6460 triple quadrupole mass spectrometer coupled with the Agilent 1200 Rapid Resolution HPLC provides highest sensitivity for small molecule profiling and MS/MS level analyses. This instrument provides extremely high sensitivity and throughput for small molecule analysis. Two such instruments are available.
Metabolite Profiling Facility (MPF)
Bindley Bioscience Center
1203 W. State St.
West Lafayette, IN 47907-2010
- (765) 494-6282
Bruce Cooper, Ph.D.
- (765) 494-6282
Amber Hopf Jannasch
- (765) 496-1756