Description

FACILITY DESCRIPTION

MPF capabilities are shared between 2 locations on the Purdue University campus:

Primary Location
Horticulture-Room 302
625 Agriculture Mall Drive
(765) 494-6282


Bindley Bioscience Center
1203 West State Street
(765) 494-6540


For MPF rates information, click this link: Rates

To schedule equipment: Researchers may either contact the MPF by phone or e-mail to schedule equipment usage. On-line instrument reservation may be made at: SCHEDULING

Facility Description

The Metabolite Profiling Facility (MPF) provides state-of-the-art technologies that enable both qualitative Metabolite (efforts to define or catalog all components of a metabolome) and quantitative Metabolite (understanding differential concentrations of metabolites) for metabolite profiling in biological systems. This facility employs highly sensitive mass spectrometry coupled with multi-dimensional gas and liquid chromatography (GC and LC-MS) to provide researchers with new technologies and methods development, as well as expert training and consultation. This research capability allows assessment of the hierarchical flow of information from the genome to the transcriptome, proteome, and metabolome ultimately defines cellular phenotypes.

How to participate

Faculty with interest and applications for Metabolite Profiling Facility (MPF) are invited to participate in the MPF Share Program. The MPF Share Program is a method of facility cost recovery that encourages:

  • Addressing larger problems associated with metabolic profiling in current research;

  • Developing protocols for the analysis of molecules of interest to the broader Purdue community; and

  • Providing students with "hands-on" experience with MS-based metabolic analysis.
Capabilities

  • High throughput metabolite profiling with LC-MS, LC/MS, MS, GC/MS or GCxGC-MS
  • Complete training in Metabolite technologies
  • Identification and characterization of small molecules
  • Automated and manual sample preparation and Solid Phase Extraction (SPE)
  • Metabolite methods development and consultation
  • Bioinformatic consultation
  • Various off-line HPLC separations and small molecule isolation
  • Automated and manual computer search and analyses