Bindley Bioscience Center

Screening assays with library of chemical compounds - Services

1. Cell-based assays (robotically)
   • distribute cell culture into 96 well plate, let cells grow in CO2 incubator;
   • add chemical compounds  and incubate for few hours;
   • wash with medium, let cells grow in CO2 incubator for 2 more days;
2. Efficiency of the tested chemicals was estimated by detection of fluorescence of targeted cells
   • fluorescence in plate (plate reader) 
   • flow cytometry in the tubes (robotically collect cells into tubes after treatment with trypsin)
   • cells viability (colorimetric MTT assay)
3. Noncell-based assays
   • Oligomerization of protein in presence of tested chemicals
   • Detection of oligomerization by running SDS gel