Bindley Bioscience Center

Screening assays with library of chemical compounds - Services

  1. Cell-based assays (robotically)
    • distribute cell culture into 96 well plate, let cells grow in CO2 incubator;
    • add chemical compounds  and incubate for few hours;
    • wash with medium, let cells grow in CO2 incubator for 2 more days;
  2. Efficiency of the tested chemicals was estimated by detection of fluorescence of targeted cells
    • fluorescence in plate (plate reader) 
    • flow cytometry in the tubes (robotically collect cells into tubes after treatment with trypsin)
    • cells viability (colorimetric MTT assay)
  3. Noncell-based assays
    • Oligomerization of protein in presence of tested chemicals
    • Detection of oligomerization by running SDS gel

Contact

Address
Purdue University
Bindley Bioscience Center
Integrated Screening Technologies
BIND-Room 222
1203 West State Street
West Lafayette, IN 47907-2057
(765) 496-2869

Larisa Avramova
Ph.D. Research Scientist