Bindley Bioscience Center

High-throughput Cloning - Services

1. Generation of PCR fragments
   • Primer design
   • Set up of PCR plate with robotic mixing of individual primers
2. Analysis of PCR fragments by agarose gel
   • video
3. Digestion with cloning enzymes
4. Ligation of fragments
5. Bacterial transformation and growth (plasmid propagation)
6. Isolation of plasmid DNA
7. Detection of plasmid concentration
8. Verification of correct plasmid by PCR or restriction analysis