Bindley Bioscience Center

Instruments

All major instruments and equipment located in this facility are outfitted to handle microplates.

• Two liquid handling dual-bridge systems (BiomekFX) containing 96- and 8-tips heads, vacuum manifold, and absorbance plate reader allow experiments to run various multisample assays.
• One of the BiomekFX systems is connected to a microplate hotel to increase the walk-away time.
• The centrifuge Qiagen K15C has a rotor for spinning the microplates.
• The Speedvac concentrator contains the plate holder.
• MJ thermocycler can run programs for 2 plates simultaneously.
• Bio-Rad iCycler is equipped with real-time PCR mode for plate.
• Genetix colony picker QPixII is a picking (from Q-trays) and replicating system.
• Accessories for bacterial and yeast cell growth include the Cryoreplicator System ‘Duetz” incubators/shakers with plate holders.
• A tissue culture room is equipped with Biosafety cabinet class II type A2, Laminar Flow and CO2 incubators.
• A liquid chromatography system AKTA Explorer100 allows fast developing and optimizing of protein purification methods.
• Spectramax384 UV/absorbance plate reader (Molecular Devices) is used for the detection of DNA and protein concentration in the plate as well as in cuvette.
• The nanodrop spectrophotometer requires only 2 ul of protein or DNA samples without dilution.
• Luminex technology is performed on BioPlex System.