Bioscience Imaging Facility

Instruments

Nikon A1R ConfocalNikon A1R Confocal

Our system features a Ti-E inverted microscope equipped with high NA oil immersion objectives, seven visible laser lines, and either four independent detectors or 32-bin spectral detection. An optional resonant scanner and piezo z-drive allow 3D image acquisition at multiple frames per second. A Tokai Hit live imaging chamber is also available (see below). NIS-Elements software encompasses acquisition through analysis, including modules for FRAP and FRET experiments.  Best performance is achieved with either cyan & yellow, or green, red, and far-red fluorophores. 

Zeiss 710 metaNikon A1R Confocal

This system features an upright microscope equipped with high NA water immmersion objectives, a motorized stage, seven visible laser lines, and a 32-window spectral detector flanked by independent PMTs. Zen software encompasses acquisition through analysis, including modules for FRAP and FRET experiments. Best performance is achieved with blue, green, red, and far-red fluorophores.

Nikon C1+ ConfocalNikon C1+ Confocal

A 90i upright microscope with a full complement of high NA oil immersion objectives, seven laser lines, up to four channel detection, and EZ-C1 software.  Cyan & yellow or blue, green, red, & far-red fluorophore combinations are possible. 

Nikon A1R MP MultiphotonNikon A1R MP Multiphoton

Our setup is built around a Ti-E inverted microscope with a ~500 um working distance 1.15 NA 40x water immersion objective. Four non-descanned detectors collect emissions excited by a Mai Tai DeepSee tunable IR laser (700-1000 nm). Two visible laser lines, including a 405 nm line for photoactivation, enable limited confocal imaging. Resonant scanning is available in both multi and single photon modes.

Nikon N-STORM/N-SIM Super ResolutionNikon N-STORM/N-SIM Super Resolution

The system is built around a Ti-E inverted microscope equipped with high NA objectives, a motorized stage, piezo z-drive, and perfect focus system. 'Classical' STORM can be performed on dyes that activate with 405 nm, 488 nm, or 561 nm light. A 647 nm laser is available to drive the reporter to the dark state or to perform dSTORM using AlexaFluor647. SIM can be performed with blue, green, or red dyes. The TIRF capability uses the STORM lasers and high-speed, multi-color TIRF (10 ms/channel) is possible using triggered acquisition.

Nikon Wide-FieldNikon WideField

A Ti-S inverted microscope equipped with objectives suitable for phase contrast or epifluorescence microscopy. Detectors include a DS-Ri1 color camera for standard applications, or alternatively, a Photometrics QuantEM:512SC CCD for imaging extremely weak signals and/or large dynamic ranges at up to 100s of frames per second.

Caliper IVIS Lumina IILumina II

The Lumina in vivo optical imager captures fluorescent or bioluminescent signals emanating from within an organism at macroscopic resolutions. Excitation and emission filters span the 450-875 nm range. The system's high sensitivity, large dynamic range camera also allow it to be used as a fluorescent plate reader or to quantify chemiluminescent bands on blotting membranes.  Living Image software enables background subtraction, automated acquisition and quantification, as well as common image processing routines.

MILabs U-SPECT-II/CTMILabs U-SPECT-II/CT

MILabs first combined single photon emission CT / x-ray CT, and the first of its kind in the USA. This newly developed system features novel multi-pinhole collimators designed for either high resolution (0.35 mm) or high sensitivity (kBeq) SPECT imaging within a 15 mm diameter field of view.  A translating robotic stage permits automatic co-registration of SPECT and CT images. Acquisition software allows post hoc sorting of incident photon data for energy thresholding, multi-channel acquisition, or temporal binning relative to biological triggers. An OS-EM reconstruction algorithm outputs NIFTI format 3-D images.

Accessories

Microscopy: A Tokai Hit (INU-TIZ-F1) live imaging chamber and 5%CO2 are available for use with any of the inverted microscopes. A cell culture hood and 5%CO2 incubator are provided for short term culture storage and basic manipulations. NIS-Elements and Image J are supported and available for image analysis.

Whole Animal Imaging: A Univentor isofluorane vaporizer unit, ECG, respiratory monitor, temperature probe and BioVet software are available for anesthesia and vitals monitoring during imaging sessions. Allentown ventilated mouse racks are available on-site for long-term animal housing during longitudinal studies. PMOD software is supported and available for analysis of SPECT/CT data.


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