Bindley Bioscience Center

Instruments

BD FACSAria III Cell Sorter

FACSAria III

The BD FACSAria III cell sorter can mount up to six lasers so that you can choose a configuration that meets your application. Wavelength choices now include the 561-nm and 445-nm lasers, as well as the 488-nm, 633-nm, 405-nm, and 375-nm lasers. The system allows you to mount up to 20 detectors and measure a maximum of 18 colors simultaneously. With the advanced gel-coupled cuvette and octagon- and trigon-shaped detector arrays that maximize signal detection, the BD FACSAria III represents the state of the art in cell sorting.

Beckman Coulter Quanta SC

Quanta

The Quanta SC is a flow cytometry analyzer with two excitation wavelengths, 488nm and a mercury arc lamp that can measure up to 3 colors simultaneously depending on which light source is turned on. This cytometer is capable of 'walk-away' operation. It runs samples in 96 well plates, sample cups, or 1.5 ml eppendorf tubes. This instrument is used for immunophenotyping, DNA analysis, cell cycle, and functional assays.

Beckman Coulter FC500

Beckman Coulter

The FC500 is a flow cytometry analyzer with two laser excitation wavelengths, 488nm and 633nm that can measure up to 5 colors simultaneously. This cytometer is capable of 'walk-away' operation. It runs samples in 96 well plates or 12x75 tubes. This instrument is used for immunophenotyping, phophoprotein analysis, DNA analysis, cell cycle, and functional assays.

iCyt Reflection Sorter

icyt reflection

The iCyt Reflection high speed cell sorter provides modern advances with proven technology. Our instrument features a dual HAPS system. HAPS 2 is a 4 laser, 10 color instrument. HAPS 3 is a 3 laser, 7 color instrument. We typically sort using a 100um nozzle but can tailor this to fit your application.

The iCyt reflection instrument is equipped with a 405nm laser, 488nm laser, 532nm laser, 647nm laser. This instrument utilizes WinList software for analysis and data acquisition. The Reflection Cell Sorter is able to sort from a 12 X 75mm polystyrene tube into multiple formats including up to four 12 X 75mm tubes, two 15mL conical tubes, or one 50mL conical tube. This is instrument is equipped with a plate sorter that can accommodate 96 well plate formats.

Cyntellect LEAP

LEAP

The LEAP (Laser Enabled Analysis and Processing) is a microplate-based, interactive image cytometer with laser photoablative sorting of either attached or suspension cells.

For more information about LEAP please read the article "Scanning Cytometry with a LEAP".


Address
Bindley Bioscience Center -- Room 233
Purdue University
West Lafayette, IN 47907-1971

Flow Cytometry Facility