Bindley Bioscience Center

Flow Cytometry & Cell Separation Facility

The Bindley Bioscience Flow Cytometry and Cell Separation Facility provides advanced cell and particle analysis and sorting using flow cytometry based technology. The facility also provides training in flow cytometry analysis and sorting techniques. The facility also provides expert consultation for assay and protocol development for flow cytometry analysis and cell separation.

BD FACSAria III Cell Sorter

The BD FACSAria III Cell Sorter fluidics system is pressure driven. Positive air pressure forces sample cells through an optically gel-coupled cuvette flow cell. Hydrodynamic focusing guides particles in a single-file stream through the cuvette, where laser light intercepts the stream at the sample interrogation point. In addition to other benefits, the next generation flow cell in the BD FACSAria III is designed to improve resolution for side population applications and DNA cell cycle analyses. With high-performance sorting and high-performance analysis FACSAria III analysis performance is comparable to state-of-the-art highly sensitive analyzers.

Beckman Coulter Quanta SC

The Quanta SC is a flow cytometry analyzer with two excitation wavelengths, 488nm and a mercury arc lamp that can measure up to 3 colors simultaneously depending on which light source is turned on. This cytometer is capable of 'walk-away' operation. It runs samples in 96 well plates, sample cups, or 1.5 ml eppendorf tubes. This instrument is used for immunophenotyping, DNA analysis, cell cycle, and functional assays

Beckman Coulter FC500

The FC500 is a flow cytometry analyzer with two laser excitation wavelengths, 488nm and 633nm that can measure up to 5 colors simultaneously. This cytometer is capable of 'walk-away' operation. It runs samples in 96 well plates or 12x75 tubes. This instrument is used for immunophenotyping, phophoprotein analysis, DNA analysis, cell cycle, and functional assays

Sony iCyt Reflection Sorter

The Sony iCyt Reflection cell sorter and analyzer provides modern advances with proven technology. This is the first cell sorter that allows for optical flexibility of up to 48 PMTs and 256 parameters, maximum performance potential with up to four HAPS (Highly Automated Parallel Sorters) in one unit. Our instrument features a dual HAPS system. HAPS 2 is a 4 laser, 10 color instrument. HAPS 3 is a 3 laser, 7 color instrument

Cyntellect LEAP

The LEAP (Laser Enabled Analysis and Processing) is a microplate-based, interactive image cytometer with laser ablative sorting of either attached or suspension cells

LEAP Case Study: In Situ purification of human embryonic stem cell colonies

LEAP Case Study: Stem Cell Colony Purification Powered by LEAP™ In situ Laser-Based Isolation of Specific Stem Cell Colonies

Image of Lab
Image of Lab

Flow Cytometry Expertise

  • DNA cell cycle analysis
  • Immunophenotyping
  • Intracellular flow cytometry
  • Antibody binding evaluation
  • Phospho-protein analysis
  • Functional flow cytometry assay development
  • Multi-parameter data analysis
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Schedule an Appointment

For more information about scheduling an appointment in the Flow Cytometry lab,

View the Scheduling page

Address
Bindley Bioscience Center -- Room 233
Purdue University
West Lafayette, IN 47907-1971

Flow Cytometry Facility

James Leary