Instruments

NEW! Q-Exactive Orbitrap HF MS (Thermo Scientific)

Used with Dionex UltiMate 3000 Nano LC system. The Thermo Scientific Q-Exactive high-field (HF) mass spectrometer combines high-resolution accurate mass (HR/AM) Orbitrap mass analyzer with a high performance quadrupole precursor ion selection to deliver unparalleled speed and sensitivity. This benchtop mass spectrometer is coupled with the Thermo Scientific UltiMate 3000 nano flow liquid chromatography (LC) system for LC-MS/MS analysis. It is highly suitable for a large scale proteomic profiling experiments. The combination of high scan speed, high resolving power and mass accuracy ensures high quality and reproducible data. It is routinely used for high-throughput characterization of proteins from a complex mixture including identification, quantitation, post-translational modification and protein-protein interaction. With the scan speed of up to 18 Hz, dynamic range of 106 and resolving power of up to 240k (FWHM) at m/z 200, this instrument significantly increases the capability to identify thousands of low abundant proteins from a single experiment. 

NEW! TSQ Endura Triple Quadrupole MS (Thermo Scientific)

Used with Dionex UltiMate 3000 Nano LC system. The Thermo Scientific TSQ Endura LC-MS is designed for targeted proteomic analysis with highest sensitivity, accuracy, and robustness. This MS comprises three quadrupoles for fast full-scan MS and MS/MS analysis of peptides, and is suitable for ultrafast selected-reaction monitoring (SRM)-assays for absolute protein quantitation. The scan speed of up to 15,000 Da/sec and linear dynamic range of up to 106, allows for sensitive, robust and routine quantitative measurement. This instrument is coupled with the Thermo Scientific UltiMate 3000 nano LC system for LC-MS/MS analysis. SRM assays using this platform allow for the absolute and highly accurate quantitation of hundreds of proteins and biomarkers in a single experiment, and requires very small amount of sample. 

4800 MALDI TOF/TOF MS (Sciex)

The advantages of MALDI TOF/TOF MS include sensitivity into the picomole range and high mass accuracy. In addition, since there is usually only a singly charged ion formed, it is a good choice for the analysis of heterogeneous samples. Furthermore, the static nature of the sample allows for multiple evaluations on a single sample. 

LTQ Orbitrap XL MS (Thermo Scientific) 

Used with Agilent 1100 Nano LC system. The Orbitrap is a sub-dalton-resolution proprietary ion trap configuration instrument that enables very high resolution analysis of peptides, post-translationally modified peptides, as well as small molecule detection and quantification. The Orbitrap rivals FT-ICR mass spectrometers with an easy-to-use and easy-to-maintain setup.

Triple TOF 5600+ MS (Sciex)

Used with Eksigent 425 Nano LC systemThe Sciex Triple TOF 5600+ System is the instrument of choice for high-sensitivity and high-resolution applications. With its fast acquisition speeds of up to 10 msec accumulation time, the TripleTOF can handle comprehensive MS/MS quantitation for proteomics applications. The instrument consistently delivers high resolution for high-speed chromatography with up to 100 spectra in a second — even in Information-Dependent Acquisition (IDA) mode. The TripleTOF is a superior instrument that is used for routine accurate mass determination where low abundance analytes are easily discovered, identified and quantified.

Contact

Uma Aryal
Proteomics Facility Director
Phone: (765) 494-4960
Email: uaryal@purdue.edu

Purdue University, 610 Purdue Mall, West Lafayette, IN 47907, (765) 494-4600

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