Instruments

NEW! Agilent 6545 Q-TOF

This UPLC/MS/MS instrument consists of an Agilent 1290 Infinity II UPLC, coupled to an Agilent 6545 quadrupole time-of-flight tandem mass spectrometer.  This instrument offers high resolution mass accuracy over a wide dynamic range, with a mass accuracy typically less than 5 ppm.  Non-targeted (discovery) experiments can be performed.  The MS/MS capability offers improved compound identification.  Comparative analysis will be performed using Agilent's Mass Profiler Professional bioinformatics software.

NEW! Thermo TSQ GC/MS/MS

A Thermo TSQ 8000 triple quadrupole mass spectrometeris coupled with a Thermo Trace 1310 GC.  Ionization is performed using either electron impact (EI) or chemical ionization (CI).  A RSH autosampler is employed, enabling either liquid or headspace sample analysis.  Acquisition and data analysis is performed using Chromeleon software.  Detection limits of ppb are possible. 

Agilent 6410 NanoLC QQQ

The Agilent 6410 Triple Quad provides high-level sensitivity for selected ion monitoring and multiple reaction monitoring for biomarker quantification studies. The HPLC system offers nano and microliter flow rates.  The 6410 includes both ESI and photoionization capability and has an onboard chip cube nanoLC system. 

Agilent HPLC/UV

A stand-alone Agilent 1100 HPLC system supports both ultraviolet and fluorescence detection. The HPLC pump is a binary system capable of up to a 10 ml/min flow rate and 400 bar of pressure. The UV-vis detector is a diode array, with a wavelength range from 190-950 nm. The fluorescence detector provides simultaneous quantitative and qualitative information for up to four signals simultaneously. The system also incorporates a mobile phase degasser, column heater and 100 vial autosampler. Data acquisition and analysis is performed using ChemStation software.  

Agilent 6460 QQQ LC/MS/MS

The Agilent 6460 triple quadrupole mass spectrometer coupled with the Agilent 1200 Rapid Resolution HPLC provides highest sensitivity for small molecule profiling and MS/MS level analyses. This instrument provides extremely high sensitivity and throughput for small molecule analysis. Two such instruments are available.

Thermo LTQ Orbitrap XL

Used with Agilent 1100 Nano LC system. The Orbitrap is a sub-dalton-resolution proprietary ion trap configuration instrument that enables very high resolution analysis of peptides, post-translationally modified peptides, as well as small molecule detection and quantification. The Orbitrap rivals FT-ICR mass spectrometers with an easy-to-use and easy-to-maintain setup.

Contact

Amber Hopf Jannasch
Metabolomics Research Scientist
Phone: (765) 496-7028
Email: hopfas@purdue.edu

Purdue University, 610 Purdue Mall, West Lafayette, IN 47907, (765) 494-4600

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