Instruments

NEW! Wyatt DynaPro Plate Reader II

The Wyatt DynaPro Plate Reader II is a high-throughput, automated dynamic light scattering instrument equipped with an onboard laser and camera system for analyzing protein, nanoparticle, colloid, and macromolecular samples in the subnanometer – few micrometer range that has been mounted in 96, 384, or 1536 well plates. The device measures size and size distributions of small sample volumes from 4 °C to 85 °C. The Wyatt DynaPro Plate Reader II enables the automatic collection of data from multiple repeat runs over a wide range of conditions to quickly determine the stability and susceptibility to aggregation, with the potential for a 35-fold acceleration of data collection speed and a high throughput ability. The fast data collection, plate reading ability, and high throughput are particularly useful for experimental systems involving protein preparations for crystallization trials or NMR analysis; nanoparticle preparations for drug delivery studies, drug-ligand conjugates, and vaccine development; and colloidal stability kinetic analyses. 

AUC (ProteomeLab XLI/XLA)

Analytical Ultracentrifugation (AUC) is a powerful tool to characterize macromolecules and study macromolecular interactions in solutions without modifications or matrices. There are two ways to study molecular sedimentation with AUC by sedimentation velocity (SV) and sedimentation equilibrium (SE). The sedimentation velocity monitors the movement of molecular boundaries under a centrifugal field, which provides hydrodynamic information, like the shape and molecular weight; and the sedimentation equilibrium analyzes the molecular distribution after reaching an equilibrium, which directly measures the average buoyant molecular weight.  

Now the BAL houses two Beckman Coulter AUC with interference and absorbance detectors, which can be used to study the quaternary structures of proteins, size distribution, binding constants, stoichiometry, and so on. 

SEC-MALS (miniDAWN TREOS + Optilab T-rEX)

Size Exclusion Column (SEC) coupled with a Multi-Angle Light Scattering (MALS) directly measure the molar mass without a reference. The SEC-MALS can be used to characterize proteins, polymers, and particles.

The Wyatt MALS in the BAL can be used to measure proteins or particles in the molar mass ranging from 200 Da to 10 MDa in ambient temperature.

ITC (MicroCal iTC200)

ITC measures the heat change of a reaction, which can be used to study the molecular interactions without labeling and modifications in a solution. The binding affinity, enthalpy change, and reactants molar ratio can be calculated from ITC results. The ITC can provide a thorough thermodynamic analysis of a reaction.

The BAL houses a Microcal iTC200, which can be used to study the molecular interactions and enzyme kinetics in low volume and high sensitivity.

BLI (Octet RED384)

BioLayer Interferometry (BLI) technique measures molecular interactions with a fiber-optic tube. The kinetics of interactions are monitored by the interference pattern of light reflected from a reference surface and a sensor surface, which the ligand is immobilized on. The interference pattern changes with analytes binding to or dissociating from the ligand.

The BLI can be used to quantify a component concentration and analyze the kinetic of interactions. The BLI is flexible in sample requirements. The buffer containing DMSO, cell lysates, and biological fluids are all compatible with BLI techniques.

The BAL houses an Octet RED 384, which has 16 channels to improve sensitivity and high throughput ability.  The sensitivity, flexibility, and high throughput make the Octet RED 384 a favorite in binding assays.

SPR (Biacore 3000)

The Biacore 3000 is a versatile tool in life sciences. By using the Surface Plasmon Resonance (SPR) technique, the Biacore 3000 can determine the binding kinetics without labeling. The Biacore 3000 can be used as an orthogonal method to Octet RED 384

Cary Eclipse Fluorimeter

The Cary Eclipse fluorimeter can be used for a variety of fluorescent-based assays. This instrument is also equipped with a manual polarizer for polarization assays and a 96-well plate adapter.

Synergy Neo Plate Reader

The Synergy NEO plate reader is compatible with 1- to 1536-well plates, featuring multiple parallel detectors including UV-Vis absorbance (230-999nm), Fluorescence intensity (250-850nm monochromator, 200-850 nm filters), Luminescence (300-700 nm), Fluorescence polarization and Time-resolved fluorescence. 

Contact

Jia Ma
Biophysical Analysis Laboratory Facility Director
Phone: (765) 496-6533
Email: ma420@purdue.edu

Purdue University, 610 Purdue Mall, West Lafayette, IN 47907, (765) 494-4600

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