Bindley Bioscience Center

Welcome to the Biophysical Analysis Lab

The purpose of the Biophysical Analysis Lab (BAL) at the Bindley Biosciences Center is to provide complete biophysical analysis of macromolecules. In the lab, there are two analytical ultracentrifuges: Beckman-Coulter XLI and XLA, a Biacore 3000, an ITC, and a SEC-MALS platform. With these instruments we can determine kinetic parameters, stoichiometry of binding, size distribution, shape, oligomerization state and other biophysical measurements. Complementing these traditional biophysical methods, the BAL also provides mass spectrometric analyses at both the protein and peptide levels. The lab strives to establish long-term collaborations and deliver professional quality data that can be used for publications and grant proposals.

XLI / XLA

There are two analytical ultracentrifuges: a Beckman Coulter XLI and a Beckman Coulter XLA. The XLI is equipped with both Rayleigh Interference and absorbance optics. This provides a complete analysis both at low concentrations (~0.1 mg/ml) and samples without chromophores. The XLA is equipped with only absorbance optics and is mainly used for protein/peptide analyses. (continue)

Biacore 3000

The Biacore 3000 is built on the platform that uses the Surface Plasmon Resonance (SPR) phenomena, which can be used to monitor the refractive index of a given medium near the surface of a chip. The Biacore 3000 uses a label free system to determine the kinetic parameters (kon/koff) of biomolecular interactions. (continue)

ITC (Isothermal Titration Calorimetry)

ITC is the only instrument capable of measuring the delta H (enthalpy) of interactions. The stoichiometry along with the strength of the interaction (Kd) can be ascertained from ITC experiments. This instrument is the gold standard for pharma companies to fine tune the drug interactions. (continue)

SEC-MALS (Size Exclusion Chromatography Multi-Angle Light Scattering)

SEC- MALS experiments can provide information on the absolute molecular weight of a system in solution without the need for standards, labeling or immobilization. (continue)

Cary Eclipse Fluorimeter

The Cary Eclipse fluorometer can be used for a variety of fluorescent based assays. The Cary Eclipse is also equipped with a manual polarizer for polarization assays and a 96-well plate adapter for larger sample analysis. (continue)

ABI 4800 MALDI TOF/TOF

The BioAnalytical Lab in collaboration with the Purdue Proteomics Facility has access to the ABI 4800 mass spectrometer. The 4800 uses matrix assisted laser desorption/ionization (MALDI) to analyze biomolecules such as proteins, peptides and sugars. With each analytical ultracentrifugation analysis, a complementary mass spectrometric whole protein analysis is conducted. (continue)

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Schedule an Appointment

For more information about scheduling an appointment in the Bioanalytical lab,

View the Scheduling page

Contact

Address
Dr. Lake Paul
Bindley Bioscience Center
Discovery Park
Purdue University
West Lafayette, IN 47907-1971