Bindley Bioscience Center

Bimolecular fluorescence complementation (BiFC) Workshop

When: 9 am - 4 pm, December 7^th, 2010

Where: Hall for Discovery and Learning Research (DLR), room 131; BBC 133/134

Registration Required: Luanne Ludwig at lml@purdue.edu or 42276 by Friday, December 3^rd.

Morning Lectures

9:00-9:45am                BiFC to image molecular interactions:  Principle, experimental design and data analysis

Chang-Deng Hu, Ph.D., DLR, room 131

10:00-10:45am            Visualization of GPCR oligomerization in living cells using BiFC-based technologies

                                    Val Watts, Ph.D., DLR, room 131

11:00-11:45am            BiFC to image protein-protein interactions in plants

                                    Stan Gelvin / Lan-Ying Lee, Ph.D., DLR, room 131

12:00-1:00pm             Lunch  (to be provide), BBC, room 221

Afternoon Labs

1:00-3:00pm               Lab Module 1 (Lan-Ying Lee): Production and transfection of Arabidopsis protoplasts for BiFC    BBC 133/134

3:00-4:00pm               Lab Module 2 (Chang-Deng Hu): Visualization and imaging of protein-protein interactions in living cells using BiFC, BBC 158 (Aaron runs microscope)